Producción Científica

Publicaciones Recientes

Di Maggio LS, Tirloni L, Uhl M, Carmona C, Logullo C, Mulenga A, da Silva Vaz I Jr , Berasain B. Serpins in Fasciola hepatica: insights into host-parasite interactions. Int J Parasitol (2020), 50(12):931-943. doi: 10.1016/j.ijpara.2020.05.010

Cancela M and Maggioli G. Cloning and heterologous expression of protein-coding sequences in Escherichia coli. Methods Mol Biol (2020), 2137:51-66. doi: 10.1007/978-1-0716-0475-5_5 

Maggioli G, Salazar C, Fossa F and Carmona C. Liver fluke vaccine assessment in cattle. Methods Mol Biol (2020), 2137:205-212. doi: 10.1007/978-1-0716-0475-5_15

Salazar C, Tort JF and Carmona C. Design of a peptide–carrier vaccine based on the highly immunogenic Fasciola hepatica leucine aminopeptidase. Methods Mol Biol (2020), 2137:205-212. doi: 10.1007/978-1-0716-0475-5_14. 

Di Maggio LS, Tirloni L, Pinto AFM, Diedrich JK, Yates JR 3rd, Carmona C, Berasain P, da Silva Vaz I Jr. A proteomic comparison of excretion/secretion products in Fasciola hepatica newly excysted juveniles (NEJ) derived from Lymnaea viatrix or Pseudosuccinea columella. Exp Parasitol (2019), 201:11-20. doi: 10.1016/j.exppara.2019.04.004.

Dominguez MF, González-Miguel J, Carmona C, Dalton JP, Cwiklinski K, Tort J, Siles-Lucas. Low allelic diversity in vaccine candidates genes from different locations sustain hope for Fasciola hepatica immunization. M Vet Parasitol (2018), 15;258:46-52. doi: 10.1016/j.vetpar.2018.06.011.

Maggioli G, Rinaldi G, Giaudrone I, Berasain P, Tort JF, Brindley PJ, Carmona C. Expression, purification and characterization of two leucine aminopeptidases of the blood fluke, Schistosoma mansoni. Molecular & Biochemical Parasitology (2018). 219, 17-23. 

McNulty SN, Tort JF, Rinaldi G, Fischer K, Rosa BA, Smircich P, Fontenla S, Choi YJ, Tyagi R, Hallsworth-Pepin K, Mann VH, Kammili L, Latham PS, Dell’Oca N, Dominguez F, Carmona C, Fischer PU, Brindley PJ, Mitreva. Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu Fevers. M PLoS Genet (2017), 13(1):e1006537. doi: 10.1371/journal.pgen.1006537.

Noya V, Brossard N, Rodríguez E, Dergan-Dylon LS, Carmona C, Rabinovich GA, Freire T. A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity. Sci Rep (2017), 7:40615. doi: 10.1038/srep40615. 

Di Maggio LS, Tirloni L, Pinto AF, Diedrich JK, Yates Iii JR, Benavides U, Carmona C, da Silva Vaz I Jr, Berasain. Across intra-mammalian stages of the liver fluke Fasciola hepatica: a proteomic study. P Sci Rep (2016), 6:32796. doi: 10.1038/srep32796.

Maggioli G, Bottini G, Basika T, Alonzo P, Salinas G, Carmona C. Immunization with Fasciola hepatica thioredoxin glutathione reductase failed to confer protection against fasciolosis in cattle. Veterinary Parasitology (2016), doi:10.1016/j.vetpar.2016.05.007.

Noya V, Brossard N, Berasaín P, Rodríguez E, Chiale C, Mazal D, Carmona C, Freire T. A mucin-like peptide from Fasciola hepatica induces parasite-specific Th1-type cell immunity. Parasitol Res (2016), 115(3):1053-63. doi: 10.1007/s00436-015-4834-z.

Cancela M, Santos GB, Carmona C, Ferreira HB, Tort JF, Zaha A. Fasciola hepatica mucin-encoding gene: expression, variability and its potential relevance in host-parasite relationship. Parasitology (2015), 142(14):1673-81. doi: 10.1017/S0031182015001134.

Rodríguez E, Noya V, Cervi L, Chiribao ML, Brossard N, Chiale C, Carmona C, Giacomini C, Freire T. Glycans from Fasciola hepatica Modulate the Host Immune Response and TLR-Induced Maturation of Dendritic Cells. PLoS Negl Trop Dis (2015), 9(12):e0004234. doi: 10.1371/journal.pntd.0004234.

Ross F, Hernández P, Porcal W, López GV, Cerecetto H, González M, Basika T, Carmona C, Maggioli G, Bonilla M, Gladyshev VG, Boiani M, Salinas G. Identification of thioredoxin glutathione reductase inhibitors that kill cestode and trematode parasites. PLoS ONE (2012), 7(4): e35033. doi:10.1371/journal.pone.0035033.

Maggioli G, Acosta D, Silveira F, Rossi S, Giacaman S, Basika T, Gayo V, Rosadilla D, Roche L, Tort J, Carmona C. rFhLAP, a recombinant gut-associated M17 leucine aminopeptidase confers high level of protection against Fasciola hepatica infection in sheep using different adjuvants. Vaccine (2011), 29:9057-9063. 

Maggioli G, Silveira F, Martín-Alonso JM, Salinas G, Carmona C, Parra F. A recombinant thioredoxin-glutathione reductase from Fasciola hepatica induces a protective response in rabbits. Experimental Parasitology (2011), 129:323-330.

Cancela M, Ruétalo N, Dell’Oca N, da Silva E, Smircich P, Rinaldi G, Roche L, Carmona C, Alvarez-Valín F, Zaha A, Tort JF. Survey of transcripts expressed by the invasive juvenile stage of the liver fluke Fasciola hepatica. BMC Genomics (2010), 11:227. doi: 10.1186/1471-2164-11-227.

Publicaciones Anteriores

Carmona, C.; Smith, A.; Dowd, A.; Dalton, J. (1993) Fasciola hepatica: a cathepsin L prevents antibody-mediated eosinophil adherence to newly excysted juveniles. Molecular and Biochemical Parasitology 62, 9.

Carmona, C., Smith, A., Dowd, A., Acosta, D., Dalton, J. (1994) Neutralization of Fasciola hepatica cathepsin L by anti-cathepsin L antibodies. Parasite Immunology 16, 235.

Carmona, C.; Mc. Gonicle, M.; Dalton, J. (1994) A novel dipeptidyl peptidase is secreted by Fasciola hepatica. Parasitology 109, 113.

Dalton, J.; Dowd, A.; Carmona, C. (1994) The cathepsin L proteinases secreted by the parasite trematode Fasciola hepatica. In: Biology of Parasitism (Erlich, R. and NIeto, A. eds.), pp. 1-15 Ed Trilce, Montevideo.

Sakai, H., Malgor, R., Basmadjian, I., Oku, Y., Kamiya, M. and Carmona, C. (1995) An enzyme-linked immunosorbent assay (ELISA) for the detection of Echinococcus granulosus coproantigens in dogs. Japanese Journal of Parasitology 44. 694-699.

Calegari, L., Gezuele, E., Torres, E., Carmona, C. (1996) Botryomycosis produced by Pseudomonas vesicularis. International Journal of Dermatology 35, 817-818.

Sato, H., Kamiya, H., Grauert, M.R., Stern, D., Altamirano, Z., Perdomo, R., Carbó, A., Alvarez, C., Monti, J. Kamiya, M., C. Carmona. (1996) Comparison of serodiagnostic tests and ultrasonography for cystic hydatidosis in an epidemiological study of rural Uruguay. Journal of Parasitology 82, 852-854.

Carbó, A.,m Yodzis, S., Carter, F., Monti, J., Carmona, C., Alvarez, C., Lloyd, S., Gemmel, M., Tiscornia, E., Yarzábal, L., Perdomo. R. Clasificación de las imágenes ecográficas de la equinococosis hepatica. Síntesis 6, 111-116.

Berasaín, P., Goñí, F., Mc.Gonicle, S., Dowd, A., Dalton, J., Frangione, B., Carmona, C. (1997) Proteinases secreted by Fasciola hepatica degrade extracelullar matrix and basement membrane components. Journal of Parasitology 83, 1-5.

Perdomo, R., Alvarez, C., Monti, J., Ferreira, C., Chiesa, A., Carbó, A., Alvez, R., Grauert, R., Stern, D., Carmona, C. (1997) Principles of the surgical approach in human liver cystic echinococcosis. Acta Tropica 64, 109-122.

Malgor, R., Nonaka, N., Basmadjian, Y., Sakai, H., Carámbula, B., Oku, Y., Carmona, C., Kamiya, M. (1997) Coproantigen detection in dogs experimentally and naturally infected with Echinococcus granulosus by a monoclonal antobody-based enzyme-linked immunosorbent assay. International Journal for Parasitology 27, 1605-1612.

Piacenza, L., Acosta, D., Dowd, A., McGonicle, S., Dalton, J.P., Carmona, C. (1997) Cathepsin L proteinases secreted by Fasciola hepatica: inhibitory effect of serum from experimentally infected rabbits demonstrated by gelatin-substrate poliacrylamide gel electrophoresis. Journal of Helminthology. 71, 333-338.

Acosta, D., Goñi, F., Carmona, C. (1998) Characterization and partial purification of a leucine aminopeptidase from Fasciola hepatica. Journal of Parasitology 1-7, 1998.

Carmona, C., Perdomo, R., Carbó, A., Alvarez, C., Monti, J., Grauert, R., Stern, D., Perera, G., Lloyd, S., Bazzini, R., Gemmell, M., Yarzábal, L. (1998) Risk factors associated with human cystic echinococcosis in Florida, Uruguay: results of a mass screening study using ultrasound and serology. American Journal of Tropical Medicine and Hygiene. 58, 599-605.

Piacenza, L., Radi, R., Goñi, F., Carmona, C. (1998) CuZn superoxide dismutase activities from Fasciola hepatica. Parasitology 117, 555-562.

Carmona, C. Proteasas secretadas por Fasciola hepatica. (1998) Boletín de la RTPD Network, 1,0, 18-19.

Piacenza, L., Acosta, D., Basmadjian, I., Dalton, J.P., Carmona, C. (1999) Vaccination with cathepsin L proteinases and with leucine aminopeptidase induces high levels of protection against fascioliasis in sheep. Infection and Immunity 67, 1954-1961.

Berasain, P., Carmona, C., Frangione, B., Dalton, J., Goñi, F. (2000) Interaction of Fasciola hepatica proteases with human IgG subclasses. Basis of parasite subversion of the humoral immune response. Experimental Parasitology 94, 99-110.

Torgerson, P. R., Carmona, C. and Bonifacino, R. (2000) Estimating the economic effects of echinococcosis: Uruguay an upper middle income developing country. Annals of Tropical Medicine and Parasitology, 94, 703-713.

Alvarez, D., Medeiros, A., Míguez, M., Casaravilla, C., Malgor, R., Carmona, C., Nieto, A. Osinaga, E. (2001) O-glycosylation in Echinococcus granulosus: Identification and characterisation of the carcinoma associated Tn antigen. Experimental Parasitology, 98, 100-109.

Carnevale, S., Rodríguez, M., Guarnera, E., Carmona, C., Tanos, T. Angel, S. (2001) Inmunodiagnosis of fasciolosis using recombinant procathepsin L cystein proteinase. Diagnositic Microbiology and Infectious Disease, 41:43-49.

Touz, C., Nores, M., Slavin, I., Carmona, C., Conrad, J., Mowatt, M., Nash, T., Coronel., C., Lujan, H. (2002) The activity of a develomentally regulated cysteine proteinase is required for cyst wall formation in the primitive eukaryote Giardia lamblia. Journal of Biological Chemistry 277, 8474 – 8481.

Touz, C., Nores, M., Slavin, I., Piacenza, L., Acosta, D., Carmona, C., Luján, H. (2002) A membrane-associated dipeptidyl peptidase IV-homolog is involved in encystation-specific gene expression during Giardia differentiation. The Biochemical Journal.364, 703-710.

Bentancor, A, Piacenza, L., Carmona, C. (2002) Immunization with cathepsin L proteinases CL1 y CL2 secreted by Fasciola hepatica elicit a preferential type 1 response based on IgG2a antibodies in rats. Journal of Helminthology 76, 199-207.

Freire, T., Robello, C., Casaravilla, C., Medeiros, A., Carmona, C., Osinaga, E. (2002) Antígenos mucínicos de O-glicolisación simple: nuevas similitudes moleculares entre células cancerosas y parásitos. Actas de Fisiología de la Facultad de Medicina, 8, 89-107.

Freire, T., Casaravilla, C., Carmona, C., Osinaga, E. (2003). Mucin type O-glycosylation in Fasciola hepatica: characterization of carcinoma associated Tn and sialyl-Tn antigens and evaluation of UDP-GalNAc:polypeptide-acetylgalactosaminyltransferase activity.  International  Journal  for  Parasitology 33, 47-56.

Casaravilla, C., Malgor, R., Carmona, C. (2003) Characterization of carbohydrates of adult Echinococcus granulosus by lectin binding analysis. Journal of Parasitology. 89, 57-61.

Berasain, P., Carmona, C., Frangione, B., Cazzulo, J.J., Goñi, F. (2003) Specific cleavage sites on human IgG subclasses by cruzipain, the major cystine proteinase from Trypanosoma cruzi. Molecular and Biochemical Parasitology.

Casaravilla, C., Freire, T., Malgor, R., Carmona, C., Osinaga, E. (2003) Mucin-type O–glycosylation in helminth parasites from major taxonomic groups: evidence for widespread distribution of the tn antigen (galnac-ser/thr) and identification of udp-galnac:polypeptide n-acetylgalactosaminyltransferase activity. Journal of Parasitology.

Moreno, M., Benavídez, U., Carol, H., Rosenkranz, C., Welle, M., Carmona, C., Nieto., A., Chabalgoity, J.A. (2004) Local and systemic immune responses to Echinococcus granulosus in experimentally infected dogs. Veterinary Parasitology 119, 37-50.

Cancela, M., Carmona, C., Rossi, S., Goñi, F., Berasain, P. (2004) Purification, characterization and immunolocalization of paramyosin from the adult stage of Fasciola hepatica. Parasitology Research 92, 441-448.

Maggioli, G., Piacenza, L., Carámbula, B., Carmona, C. (2004) Purification and characterisation of a thioredoxin reductase from Fasciola hepatica. Journal of Parasitology 90, 205-211.

Oku, Y., Malgor, R., Benavidez, U., Carmona, C., Kamiya, M. (2004) Control program against hydatidosis and the decreased prevalence in Uruguay. International Congress Series 1267, 98-104.

Casaravilla, C., Malgor, R., Rossi, A., Sakai, H., Nonaka, N., Kamiya, M., Carmona, C. (2004) Production and characterization of monoclonal antibodies against excretory / secretory products of adult Echinococcus granulosus, and their application to coproantigen detection. Parasitology International online.

Casaravilla, C., Malgor, R., Rossi, A., Sakai, H., Nonaka, N., Kamiya, M., Carmona, C. (2005) Production and characterization of monoclonal antibodies against excretory / secretory products of adult Echinococcus granulosus, and their application to coproantigen detection. Parasitology International 54, 43-49.

Ubillos, L., Medeiros, A., Cancela, M., Casaravilla, C., Saldaña, Y., Domínguez, L., Carmona, C., Le Pendu, J., Osinaga, E. (2007) Characterisation of the carcinoma associated Tk antigen in helminth parasites. Experimental Parasitology 116, 129-136.

Rioux, M.C., Carmona, C., Acosta, D., Ward, B., Ndao, M., Gibbs, B., Bennett, H., Spithill, T.W. (2008) Discovery and validation of serum biomarkers expressed over the first twelve weeks of Fasciola hepatica infection in sheep. International Journal for Parasitology 38, 123-136.

Marcilla, A., De la Rubia, J. E., Sotillo, J., Bernal., Carmona, C., Villavicencio, Z., Acosta., D., Tort, J., Bornay, F.J., Esteban, J.G., Toledo, R. (2008) Leucine aminopeptidase is an immunodominant antigen of Fasciola hepatica excretory and secretory products in human infections. Clinical and Vaccine Immunology 15, 95-100.

Acosta, D., Cancela, M., Piacenza, L., Roche, L., Carmona, C., Tort, J. (2008) Fasciola hepatica leucine aminopeptidase, a promising candidate for vaccination against ruminant fasciolosis. Molecular and Biochemical Parasitology 158, 52-64.

Cancela, M., Acosta, D., Rinaldi, G., Silva, E., Duran, R., Roche, L., Zaha, A., Carmona, C., Tort, J. (2008) A distinctive repertoire of cathepsins is expressed by juvenile invasive Fasciola hepatica. Biochimie 90, 1461-1475.

Cancela, M., Ruétalo, N., Dell´Oca, N., da Silva, E., Smircich, P., Rinaldi, G., Roche, L., Carmona, C., Alvarez-Valín, F., Zaha, A., Tort, J. (2010) Survey of transcrips expressed by the invasive juvenile stage of the trematode Fasciola hepatica. BMC Genomics 11, 227.