2. Publicaciones


Di Maggio LS, Tirloni L, Uhl M, Carmona C, Logullo C, Mulenga A, da Silva Vaz I Jr Berasain B. Serpins in Fasciola hepatica: insights into host-parasite interactions. Int J Parasitol (2020), 50(12):931-943. doi: 10.1016/j.ijpara.2020.05.010

Cancela M and Maggioli G. Cloning and heterologous expression of protein-coding sequences in Escherichia coli. Methods Mol Biol (2020), 2137:51-66. doi: 10.1007/978-1-0716-0475-5_5 

Maggioli G, Salazar C, Fossa F and Carmona C. Liver fluke vaccine assessment in cattle. Methods Mol Biol (2020), 2137:205-212. doi: 10.1007/978-1-0716-0475-5_15

Salazar C, Tort JF and Carmona C. Design of a peptide–carrier vaccine based on the highly immunogenic Fasciola hepatica leucine aminopeptidase. Methods Mol Biol (2020), 2137:205-212. doi: 10.1007/978-1-0716-0475-5_14. 

Di Maggio LS, Tirloni L, Pinto AFM, Diedrich JK, Yates JR 3rd, Carmona C, Berasain P, da Silva Vaz I Jr. A proteomic comparison of excretion/secretion products in Fasciola hepatica newly excysted juveniles (NEJ) derived from Lymnaea viatrix or Pseudosuccinea columella. Exp Parasitol (2019), 201:11-20. doi: 10.1016/j.exppara.2019.04.004.

Dominguez MF, González-Miguel J, Carmona C, Dalton JP, Cwiklinski K, Tort J, Siles-Lucas. Low allelic diversity in vaccine candidates genes from different locations sustain hope for Fasciola hepatica immunization. M Vet Parasitol (2018), 15;258:46-52. doi: 10.1016/j.vetpar.2018.06.011.

Maggioli G, Rinaldi G, Giaudrone I, Berasain P, Tort JF, Brindley PJ, Carmona C. Expression, purification and characterization of two leucine aminopeptidases of the blood fluke, Schistosoma mansoni. Molecular & Biochemical Parasitology (2018). 219, 17-23. doi.org/10.1016/j.molbiopara.2017.11.006. 

McNulty SN, Tort JF, Rinaldi G, Fischer K, Rosa BA, Smircich P, Fontenla S, Choi YJ, Tyagi R, Hallsworth-Pepin K, Mann VH, Kammili L, Latham PS, Dell'Oca N, Dominguez F, Carmona C, Fischer PU, Brindley PJ, Mitreva. Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu Fevers. M PLoS Genet (2017), 13(1):e1006537. doi: 10.1371/journal.pgen.1006537.

Noya V, Brossard N, Rodríguez E, Dergan-Dylon LS, Carmona C, Rabinovich GA, Freire T. A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity. Sci Rep (2017), 7:40615. doi: 10.1038/srep40615. 

Di Maggio LS, Tirloni L, Pinto AF, Diedrich JK, Yates Iii JR, Benavides U, Carmona C, da Silva Vaz I Jr, Berasain. Across intra-mammalian stages of the liver fluke Fasciola hepatica: a proteomic study. P Sci Rep (2016), 6:32796. doi: 10.1038/srep32796.

Maggioli G, Bottini G, Basika T, Alonzo P, Salinas G, Carmona C. Immunization with Fasciola hepatica thioredoxin glutathione reductase failed to confer protection against fasciolosis in cattle. Veterinary Parasitology (2016), doi:10.1016/j.vetpar.2016.05.007. 

Noya V, Brossard N, Berasaín P, Rodríguez E, Chiale C, Mazal D, Carmona C, Freire T. A mucin-like peptide from Fasciola hepatica induces parasite-specific Th1-type cell immunity. Parasitol Res (2016), 115(3):1053-63. doi: 10.1007/s00436-015-4834-z.

Cancela M, Santos GB, Carmona C, Ferreira HB, Tort JF, Zaha A. Fasciola hepatica mucin-encoding gene: expression, variability and its potential relevance in host-parasite relationship. Parasitology (2015), 142(14):1673-81. doi: 10.1017/S0031182015001134.

Rodríguez E, Noya V, Cervi L, Chiribao ML, Brossard N, Chiale C, Carmona C, Giacomini C, Freire T. Glycans from Fasciola hepatica Modulate the Host Immune Response and TLR-Induced Maturation of Dendritic Cells. PLoS Negl Trop Dis (2015), 9(12):e0004234. doi: 10.1371/journal.pntd.0004234. 

Ross F, Hernández P, Porcal W, López GV, Cerecetto H, González M, Basika T, Carmona C, Maggioli G, Bonilla M, Gladyshev VG, Boiani M, Salinas G. Identification of thioredoxin glutathione reductase inhibitors that kill cestode and trematode parasites. PLoS ONE (2012), 7(4): e35033. doi:10.1371/journal.pone.0035033.

Maggioli G, Acosta D, Silveira F, Rossi S, Giacaman S, Basika T, Gayo V, Rosadilla D, Roche L, Tort J, Carmona C. rFhLAP, a recombinant gut-associated M17 leucine aminopeptidase confers high level of protection against Fasciola hepatica infection in sheep using different adjuvants. Vaccine (2011), 29:9057-9063. 

Maggioli G, Silveira F, Martín-Alonso JM, Salinas G, Carmona C, Parra F. A recombinant thioredoxin-glutathione reductase from Fasciola hepatica induces a protective response in rabbits. Experimental Parasitology (2011), 129:323-330.

Cancela M, Ruétalo N, Dell'Oca N, da Silva E, Smircich P, Rinaldi G, Roche L, Carmona C, Alvarez-Valín F, Zaha A, Tort JF. Survey of transcripts expressed by the invasive juvenile stage of the liver fluke Fasciola hepatica. BMC Genomics (2010), 11:227. doi: 10.1186/1471-2164-11-227.
Actividades de Investigación

1. Líneas Actuales de Investigación

  • Clonado y expresión de proteínas recombinantes de Fasciola hepatica con potencial inmunoprotector: leucinas aminopeptidasas (LAP1 y LAP2), serpinas, legumaina y paramiosina.

  • Identificación de nuevos blancos moleculares por análisis proteómico contra parásitos.

  • Desarrollo de una vacuna recombinante contra fasciolosis en rumiantes.

  • Plataforma biotecnológica para aislamiento, estudio y caracterización de vesículas extracelulares (VEs) de interés biomédico.

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INSTITUTO DE HIGIENE
 

Departamento de Biología Celular y Molecular

Instituto de Biología - Facultad de Ciencias

Unidad de Biología Parasitaria